Mass photometry is a revolutionary way to measure the mass of molecules in their native states. Early mass photometry experiments, developed and pioneered by Philipp Kukura from the University of Oxford, imaged molecules on solid glass surfaces. However, many macromolecules, such as membrane-associated proteins and integral membrane proteins, perform their most important functions and interact with reaction partners in lipid membranes. In this webinar brought to you by Refeyn, Nikolas Hundt, a former postdoctoral researcher in the Kukura laboratory, now at Ludwigs-Maximilian-University Munich, will describe a new mass photometry strategy for unlabelled molecules diffusing on supported lipid bilayers. With this approach, called mass-sensitive particle tracking (MSPT), researchers can determine the mass distributions and diffusion characteristics of membrane-associated protein complexes and observe protein assembly dynamics on a lipid interface in real time.
Nikolas Hundt will be joined by Tamara Heermann and Frederik Steiert from the laboratory of Petra Schwille at the Max Planck Institute of Biochemistry during the question-and-answer session.
Analyzing the membrane-associated Escherichia coli MinDE system with MSPT
Wednesday, June 30, 2021
11:00 AM - 12:00 PM Eastern Time
Meet the panel
Nikolas Hundt, PhD Postdoctoral Researcher Ludwig-Maximilians-University of Munich, Germany
Tamara Heermann Senior doctoral researcher Max Planck Institute of Biochemistry
Frederik Steiert PhD candidate Max Planck Institute of Biochemistry
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TS_WEBINARISGNUP_Measuring Membrane Proteins with Mass-Sensitive Particle Tracking June 23, 2021
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