From detecting trace levels of virus to quantifying oncogenes in patient samples, quantitative PCR (qPCR) is the cornerstone of many diagnostic tests. At the basic research level, scientists rely on qPCR to evaluate changes in gene expression in varying conditions. qPCR success depends on careful primer design, sample and reagent purity, template composition, and experimental technique.
This Technique Talk will teach you the tips and tricks needed for obtaining perfect qPCR results.
How to design and validate a qPCR assay
Optimize qPCR-based analysis workflows
Assess the performance of qPCR analysis
Friday, January 29, 2021
11:30 AM - 12:30 PM Eastern Time
Mikael Kubista, PhD Head, Department of Gene Expression Institute of Biotechnology, Czech Academy of Sciences Founder and CEO, TATAA Biocenter
Troubleshooting Quantitative PCR (qPCR)
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