Organoids grown from stem cells are powerful research tools, and researchers can enhance their usefulness in development and disease modeling by introducing genetic mutations. However, using traditional methods of genetic manipulation in organoids is a challenge. To successfully grow desired cell types and support organoid growth and selection after gene editing, researchers must carefully formulate conditioned media. Furthermore, scientists can prepare organoid media that acts as a selection marker after CRISPR/Cas9 editing.
This Technique Talk will teach the tips and tricks needed for growing organoids from stem cells in 3D culture conditions and how to use conditioned media for selection after CRISPR editing.
Learning Objectives
Optimize conditioned media for organoid culture
Improve primary organoid culture conditions after genetic manipulation
Overcome challenges associated with traditional cloning in organoid culture using CRISPR editing
Wednesday, March 31, 2021
11:00 AM - 12:00 PM Eastern Time
Instructor
Mahnaz Darvish, PhD Postdoctoral Research Scientist, Nuffield Department of Surgical Science University of Oxford Research Associate, Wellcome-MRC Cambridge Stem Cell Institute University of Cambridge
Joining the live Q&A
Yas Heidari, PhD Protein and Cell Culture Product Manager Bio-Techne
Kevin Flynn, PhD R&D Manager, Stem Cells Bio-Techne
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Supporting Organoid Growth in 3D Cell Culture
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