ATAC-seq experiments provide scientists with genome-wide snapshots of a cell type’s epigenetic landscape at the population or single cell level. This powerful technology probes gene regulatory networks with less input material compared to other epigenetic methodologies, such as chromatin immunoprecipitation and bisulfite sequencing. However, experimental success depends on multiple factors, including sequencing depth, sample type, and sample preparation quality.
This Technique Talk will reveal how to optimize bulk and single cell ATAC-seq experiments for various sample types to obtain high quality, reproducible data.
Learning Objectives
Preparing single cell versus bulk ATAC-seq samples
Verifying sample quality and determining sequencing depth
Identifying and avoiding common pitfalls
Thursday, February 10, 2022
3:00 – 4:00 PM Eastern Time
Instructor
Claudia Lalancette, PhD Managing Director Epigenomics Core University of Michigan
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